Protocols and Guidelines

Download our protocols and guides on how to make 3D cultures, including cell cultures and microbial cultures, seed and recover cells, and create disease models.
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How to Perform 3D Cell Culture

This protocol provides general guidelines on how to seed cells on P3D Scaffolds to perform 3D cell culturing. It is a suggestion for the 3D culturing procedure for a wide range of cells, including but not limited to, stem cells and cancer cells.


How to 3D Culture Human Mesenchymal Stem Cells

This protocol is for seeding and 3D culturing stem cells on P3D Scaffolds in a 24-well plate. It is a suggestion for the seeding procedure of stem cells; however, the procedure is based on the use of human mesenchymal stem cells (hMSCs).


Do you want to know more about applications and use cases?

P3D Scaffolds were recently utilized as tissue engineering scaffolds in an in vivo study. The purpose of the experiment was to test and develop a system for non-invasive, non-destructive, and label-free monitoring of bone tissue development, using novel spectroscopic techniques.