Protocols and Guidelines

Download our protocols and guides on how to make 3D cultures, including cell cultures and microbial cultures, and create physiologically relevant disease models.

If you have any questions, email We are always available to discuss how we can help your team develop the right 3D cell model customized to your research needs.

P3D Scaffold Quick Start Protocol

This protocol provides general guidelines on how to seed cells on P3D Scaffolds to create 3D cell cultures. It is a suggestion for the 3D culturing procedure for a wide range of cells, including but not limited to, stem cells and cancer cell lines.


How to 3D Culture Human Mesenchymal Stem Cells

This protocol is for seeding and 3D culturing stem cells on P3D Scaffolds in a 24-well plate. The procedure provides guidelines on how to create three-dimensional multicellular in vitro tissue constructs, while it is based on the use of human mesenchymal stem cells (hMSCs) that are differentiated into osteoblasts to create a 3D bone cell culture.


How to Perform Biofilm Investigations in Vitro

This guide is for seeding bacteria and thereby forming biofilm on P3D Scaffolds to mimic microbe attachment and interactions in relation to human bone and bone implants. To understand biofilm formation mechanisms, the scaffold can be used to investigate microbe attachment and how biofilm-residing cells interact with 1) each other, 2) synthetic bone implants, and 3) bone cells on and within the porous structure. The structures can further be used to test the efficacy of antimicrobial treatments in relation to biofilm prevention and treatment.


Do you want to know more about applications and use cases of the 3D cell culture system?

P3D Scaffolds were recently utilized as tissue engineering scaffolds in an in vivo study. The purpose of the experiment was to test and develop a system for non-invasive, non-destructive, and label-free monitoring of bone tissue development, using novel spectroscopic techniques.