P3D Research Line

Uniquely Lifelike Bone Environments

P3D Bone Scaffolds

Our bones are of vital importance for our health, function, and appearance. Unfortunately, a wide variety of diseases can affect our bones such as osteoporosis, osteoarthritis, bone tumors, osteomyelitis, and different forms of trauma both surgical and accidental.

Developing treatments for such diseases is of great importance and this requires bone models wherein disease mechanisms can be studied and/or new treatments can be tested.

To enable researchers to do this important work, Particle3D is now offering a porous 3D bone scaffold based upon 3D printed β-tricalcium phosphate that can be used in vitro and in vivo animal models.

Directly Translate Your Research From In Vitro to In Vivo

P3D Scaffolds can be used as physiologically relevant in vitro models as well as in in vivo animal trials. Using the same natural material and 3D structure across research methods secures optimal conditions to obtain useful and highly relevant results.

With P3D Scaffolds, the conclusions derived from your in vitro experiments accurately account for the events that occur in vivo.

Why 3D Printed Scaffolds?

Particle3D 3D prints the β-TCP structures with internal porosities to maximize the surface area onto which cells can attach, this mimics the trabecular bone structure observed in human bones. Our 3D printed structures allow researchers to add different cells and pharmaceuticals onto these structures to study how they interact with each other and the bone within its pores. For example:

  • Mesenchymal stem cells or osteoblasts can be added to study how new bone develops in bone grafts.

  • Osteoclasts and macrophages can be added to study bone destruction in osteoporosis/arthritis.

  • Cancer cells can be added to study the development of bone tumors and how they destroy bone.

  • Bacteria cells can be added to create an osteomyelitis model to study how they destroy bone and evade the immune system and pharmaceuticals.

These models may be used to study bone disease mechanisms but they can also be used to test new treatments by applying new pharmaceutical or cell therapy candidates to the cellularized structures. While this can also be done in traditional 2D culture, our 3D printed bone environment better mimics the 3D structures of bone and can be used to test the therapies in lifelike structures wherein drug perfusion is uneven and where bacteria and cancer cells may hide in pores. This allows for more realistic testing of therapies that are thus more likely to yield results when subsequently translated.

 

Why Tricalcium Phosphate?

The P3D Scaffolds are made from a nonpolymeric bioink of beta tricalcium phosphate (β-TCP) and fatty acid. Because P3D Scaffolds do not contain any materials foreign to the body, the bone scaffolds greatly remsemble native bone tissue, facilitating a realistic cell adhesion and growth.

Since β-TCP is a widely used bone graft substitute, your results will be directly relevant to the many patients that are treated with β-TCP.

(1) Fernandez de Grado G, Keller L, Idoux-Gillet Y, Wagner Q, Musset AM, Benkirane-Jessel N, et al. Bone substitutes: a review of their characteristics, clinical use, and perspectives for large bone defects management. J Tissue Eng. 2018;9:2041731418776819.

Patent status: The product is protected by one or more US, European, and/or foreign patents.

Disclaimer: The products are “For Research Use Only (RUO)” and should not be used for clinical purposes. Particle3D makes no other warranties, expressed or implied, including the implied warranty of merchantability and the implied warranty of fitness for particular purpose.