P3D Research Line

Uniquely Lifelike Bone Environments

P3D Bone Scaffolds

Our bones are of vital importance for our health, function and appearance. Unfortunately, a wide variety of diseases can affect our bones such as osteoporosis, osteoarthritis, bone tumors, osteomyelitis and different forms of trauma both surgical and accidental.

Developing treatments for such diseases is of great importance and this requires bone models wherein disease mechanisms can be studied and/or new treatments can be tested.

To enable bone researchers to do this important work, Particle3D is now offering a porous 3D bone model based upon 3D printed β-tricalcium phosphate that can be used in vitro or in in vivo animal models.

Directly Translate Your Research From In Vitro
to In Vivo

The P3D Scaffolds can be used in both in vitro and in vivo to bridge the gap between the lab and animal trial research.

This allows for better extrapolations between your laboratory research and animal trials, thereby securing that the conclusions derived from your in vitro experiments accurately account for the events that occur in vivo.

Why 3D Printed Scaffolds?

Particle3D 3D print the β-TCP structures with internal porosities to maximize the surface area onto which bone cells can attach, this mimics the trabecular bone structure observed in human bones. Our 3D printed structures allow researchers to add different cells and pharmaceuticals onto these structures to study how they interact with each other and the bone within its pores.

  • Mesenchymal stem cells or osteoblasts can be added to study how new bone develops in bone grafts.

  • Osteoclasts and macrophages can be added to study bone destruction in osteoporosis/arthritis.

  • Cancer cells can be added to study the development of bone tumors and how they destroy bone.

  • Bacteria cells can be added to create an osteomyelitis model to study how they destroy bone and evade the immune system and pharmaceuticals.

These models may be used to study bone disease mechanisms but they can also be used to test new treatments by applying new pharmaceutical or cell therapy candidates to the cellularized structures. While this can also be done in traditional 2D culture, our 3D printed bone environment mimics the 3D structures of bone and can be used to test the therapies in lifelike structures wherein drug perfusion is uneven and where bacteria and cancer cells may hide in pores. This allows for more realistic testing of therapies that are thus more likely to yield results when subsequently translated.

 

Why Tricalcium Phosphate?

Beta tricalcium phosphate (β-TCP) is the golden standard within synthetic bone graft materials (1). It is biodegradable and biocompatible and has been widely used clinically for filling and reconstructing bony defects in orthopedic surgery and dentistry.

Our bones are mostly composed of calcium and phosphate which is why we want to model and rebuild them using β-TCP ― a mineral that contains only phosphate and calcium. Since β-TCP is a widely used bone graft substitute, your results will be directly relevant to the many patients that are treated with β-TCP.

(1) Fernandez de Grado G, Keller L, Idoux-Gillet Y, Wagner Q, Musset AM, Benkirane-Jessel N, et al. Bone substitutes: a review of their characteristics, clinical use, and perspectives for large bone defects management. J Tissue Eng. 2018;9:2041731418776819.